Acetic Acid Water 0.6%

Acetic Acid 0.6% Solution

Acetic Acid 0.6% Solution delivers pharmaceutical-grade purity in a convenient, ready-to-use format. This sterile aqueous preparation combines precisely measured acetic acid with ultra-pure water to create an optimal environment for peptide solubilization, analytical chemistry, and biochemical research applications requiring controlled acidity.

Trusted by researchers worldwide, this solution eliminates preparation variability and ensures consistent, reproducible results across diverse experimental protocols.

Why Choose Acetic Acid 0.6% Solution?

Optimal for Peptide Science

The 0.6% concentration represents the ideal balance for peptide work—sufficient acidity to enhance solubility of challenging compounds while remaining gentle enough to preserve structural integrity. Many lyophilized peptides, particularly those with hydrophobic characteristics or aggregation tendencies, dissolve more readily in mildly acidic environments.

Sterile & Endotoxin-Free

Unlike standard laboratory acetic acid, our solution undergoes sterile filtration and rigorous endotoxin testing. This makes it suitable not only for analytical work but also for applications involving cell culture, tissue studies, and biological assays where contamination could compromise results.

Analytical Grade Purity

With verified purity exceeding 99.4% by HPLC and comprehensive mass spectrometry confirmation, this solution meets the stringent requirements of modern analytical techniques including LC-MS, HPLC, and spectroscopy.

Time-Saving Convenience

Receive a ready-to-use solution that eliminates the need for dilution calculations, sterile filtration, or quality verification. Simply open and use, saving valuable laboratory time while ensuring consistent concentration every time.

Technical Specifications

Chemical Profile:

• Compound: Acetic Acid (Ethanoic Acid)
• Formula: CH₃COOH
• Concentration: 0.6% volume/volume in water
• CAS Number: 64-19-7
• Molecular Weight: 60.05 g/mol

Physical Properties:

• Appearance: Clear, colorless liquid
• Odor: Characteristic mild vinegar odor
• pH: 3.5-4.0 (typical)
• Specific Gravity: ~1.001 at 20°C
• Miscibility: Fully miscible with water and many organic solvents

Quality Metrics:

• HPLC Purity: 99.42%
• MS Verification: 711.9 Da (observed)
• Retention Time: 3.48 min (LCMS-7800)
• Impurity Profile: <0.58% total
• Sterility: Passes USP <71> sterility test
• Endotoxins: <0.5 EU/mL by LAL assay
• Particulate Matter: Passes USP <788> standards

Packaging Options:

• 10 mL amber glass bottle with sterile cap
• 30 mL amber glass bottle with sterile cap
• 50 mL amber glass bottle with sterile cap
• 100 mL option for high-volume users
• Custom volumes available for institutional purchasers

Research Applications

Peptide Reconstitution & Solubilization

Primary Application: Dissolving lyophilized peptides that are difficult to solubilize in neutral water. The mild acidity protonates basic amino acid residues, increasing net charge and electrostatic repulsion between molecules—reducing aggregation and improving dissolution.

Best For:

• Hydrophobic peptides with low water solubility
• Peptides with tendency to aggregate or precipitate
• Modified peptides (acetylated, amidated, pegylated)
• Peptides requiring acidic conditions for stability
• Membrane-associated peptide sequences

Typical Protocol: Add solution slowly to lyophilized peptide while gently swirling. For stubborn peptides, brief sonication (5-10 seconds) in ice bath may assist dissolution. Once dissolved, can be further diluted with buffer or media as needed.

Analytical Chemistry & Chromatography

HPLC Mobile Phase Component: Widely used in reversed-phase HPLC as a mobile phase additive. The acetic acid suppresses ionization of acidic compounds, improves peak shape, and enhances reproducibility. Compatible with UV, fluorescence, and mass spectrometry detection.

Sample Preparation: Serves as diluent for samples requiring acidic pH before analysis. Prevents adsorption of peptides to container walls and maintains analyte stability during preparation and storage.

Mass Spectrometry: Volatile nature of acetic acid makes it MS-compatible. Enhances ionization efficiency for certain compounds while maintaining signal cleanliness without non-volatile salt contamination.

Biochemical Assays & Enzymatic Studies

pH Adjustment: Provides gentle acidification for reactions requiring mildly acidic conditions. Lower ionic strength compared to traditional buffers makes it suitable for assays where salt interference is problematic.

Enzyme Kinetics: Used as vehicle or diluent in enzyme studies, particularly for enzymes with acidic pH optima or substrates requiring acidic solubilization.

Protein Stability Studies: Investigates protein behavior under mildly acidic stress conditions, relevant for understanding pharmaceutical formulation stability and storage.

Cell Biology & Tissue Research

Fixation Protocols: Acetic acid is a component in certain fixation solutions (e.g., Carnoy's fixative, methanol-acetic acid). This sterile preparation ensures contamination-free fixation for sensitive applications.

Cell Permeabilization: Mild acetic acid solutions can facilitate cell membrane permeabilization in specific protocols while maintaining low toxicity.

Decalcification: Used in some tissue preparation protocols for gentle decalcification of bone or calcified tissue samples.

Step-by-Step Usage Guide

For Peptide Reconstitution

Step 1: Preparation

• Remove lyophilized peptide from freezer
• Allow vial to equilibrate to room temperature (10-15 minutes)
• This prevents condensation which could affect concentration

Step 2: Calculate Volume

• Determine desired final concentration
• Calculate volume needed: Volume (mL) = Peptide mass (mg) ÷ Desired concentration (mg/mL)

Step 3: Aseptic Transfer

• Clean peptide vial stopper with 70% alcohol
• Using sterile syringe and needle, withdraw calculated volume
• Slowly dispense solution along vial wall (not directly onto peptide)

Step 4: Dissolution

• Gently swirl or invert vial—do not vortex vigorously
• Allow 2-5 minutes for complete dissolution
• If cloudiness persists, gentle sonication may help

Step 5: Storage or Use

• Use immediately for experiments, or
• Store according to peptide-specific requirements
• Most peptide solutions stable 7-30 days at 2-8°C

For Analytical Applications

HPLC Mobile Phase:

• Typical concentration: 0.1-1.0% acetic acid in mobile phase
• Dilute stock solution with HPLC-grade water or acetonitrile
• Degas before use to remove dissolved gases
• Filter through 0.22 μm membrane if particulates present

Sample Dilution:

• Use directly or dilute as needed
• Mix thoroughly after dilution
• Centrifuge if precipitate forms (10,000 x g, 5 min)

Storage & Stability

Optimal Storage Conditions

Unopened Bottles:

• Temperature: 15-25°C (room temperature)
• Location: Cool, dry place away from direct sunlight
• Orientation: Upright to prevent cap contact with solution
• Shelf Life: 24 months from manufacturing date

Opened Bottles:

• Reseal tightly immediately after each use
• Store at room temperature or refrigerate (2-8°C)
• Use within 6 months of opening
• Monitor for any cloudiness or color change

Preventing Contamination

Critical Practices:

• Always use sterile syringes and needles for withdrawal
• Never pour solution and return excess to bottle
• Do not introduce pipette tips directly into stock bottle
• Consider aliquoting into smaller sterile containers for routine use
• Label aliquots with date opened and expiration

Signs of Contamination:

• Cloudiness or turbidity
• Color change (yellowing)
• Particulate matter or floating debris
• Unusual odor (beyond normal acetic acid smell)
• If any observed, do not use—discard properly

Environmental Considerations

Temperature Sensitivity:

• Stable across normal laboratory temperatures (15-30°C)
• Freezing not recommended (may cause container damage)
• Avoid storage near heat sources or in direct sunlight

Light Protection:

• Amber glass bottles provide UV protection
• Additional light protection generally not necessary
• For long-term storage, dark cabinets preferable

Quality Control & Certification

Comprehensive Testing Protocol

Every production batch undergoes multi-point quality verification:

Identity Confirmation:

• Mass spectrometry (MS) confirms molecular identity
• Retention time matches reference standard (±0.05 min)
• Chemical fingerprint verified by HPLC-UV

Purity Assessment:

• High-performance liquid chromatography quantifies purity
• Multiple detection methods (UV, ELSD) for comprehensive analysis
• Impurity profiling identifies any contaminants

Sterility Assurance:

• Sterile filtration through 0.22 μm membrane
• USP <71> sterility testing on finished product
• Incubation in multiple media types to detect aerobic, anaerobic, and fungal contamination

Endotoxin Testing:

• Limulus Amebocyte Lysate (LAL) assay
• Specification: <0.5 EU/mL
• Critical for cell culture and biological applications

pH Verification:

• Measured using calibrated pH meter
• Specification: 3.4-4.1
• Ensures consistency for pH-sensitive applications

Particulate Matter:

• Visual inspection under controlled lighting
• Microscopic examination if needed
• Meets USP <788> requirements

Certificate of Analysis

Each batch includes comprehensive documentation:

• Batch/lot number and manufacturing date
• Complete test results with specifications
• Instrument calibration information
• Approved by QC manager signature
• Traceability to raw materials
• Available digitally or as hard copy

Comparison with Alternatives

vs. Standard Laboratory Acetic Acid

Sterility:

• Standard: Non-sterile, contains microorganisms
• Our Solution: Sterile-filtered, suitable for biological work

Purity:

• Standard: Variable, typically 95-99%
• Our Solution: >99.4% verified by HPLC

Consistency:

• Standard: Batch-to-batch variation common
• Our Solution: Tight specifications, reproducible results

Convenience:

• Standard: Requires dilution calculation and preparation
• Our Solution: Ready-to-use, no preparation needed

vs. DIY Dilution from Glacial Acetic Acid

Safety:

• Glacial: Highly corrosive, requires careful handling
• Our Solution: Mild, safer to handle

Accuracy:

• Glacial dilution: Potential for calculation or pipetting errors
• Our Solution: Precisely formulated, verified concentration

Time:

• DIY: Requires preparation time, sterile filtration, quality check
• Our Solution: Immediate use, saves 30-60 minutes per preparation

Cost-Effectiveness:

• DIY: Cheaper per mL but requires labor, equipment, quality control
• Our Solution: Higher per-mL cost offset by convenience and reliability

Safety Information

Hazard Classification

While 0.6% acetic acid is relatively mild, proper laboratory safety practices should always be followed:

Physical Hazards:

• Non-flammable at this concentration
• No explosion or reactive hazards

Health Hazards:

• Eye contact: May cause irritation
• Skin contact: May cause mild irritation with prolonged exposure
• Inhalation: May irritate respiratory tract
• Ingestion: May cause stomach irritation (NOT for human consumption)

Personal Protective Equipment

Minimum Required:

• Safety glasses or goggles
• Nitrile or latex gloves
• Laboratory coat or protective clothing

Enhanced Protection (for large volumes):

• Face shield in addition to safety glasses
• Work in fume hood if extensive handling
• Chemical-resistant apron

Emergency Procedures

Eye Contact:

• Immediately flush with water for 15 minutes
• Hold eyelids open to ensure thorough rinsing
• Seek medical attention if irritation persists

Skin Contact:

• Wash affected area with soap and water
• Remove contaminated clothing
• If irritation develops, seek medical advice

Inhalation:

• Move to fresh air immediately
• If breathing difficulty occurs, seek medical attention

Ingestion:

• Rinse mouth with water
• Do NOT induce vomiting
• Seek immediate medical attention
• Provide SDS to medical personnel

Disposal

Small Quantities:

• Can typically be disposed via sanitary sewer with water flush
• Check local regulations first

Large Quantities:

• Collect in appropriate waste container
• Neutralize with sodium bicarbonate if required
• Dispose as acidic aqueous waste per institutional protocols

Scientific Background

Regulatory Standards Compliance

This formulation aligns with established guidelines from leading scientific and regulatory organizations:

U.S. Pharmacopeia (USP): Standards for water for pharmaceutical purposes, solvent purity, and sterility testing methodologies inform our manufacturing protocols.

Centers for Disease Control (CDC): Laboratory water quality control guidelines ensure our production processes meet microbiological safety standards.

International Organization for Standardization (ISO): ISO standards for water for injection and laboratory solvents guide our quality management systems.

Editorial Oversight

Technical documentation compiled by the Research ROI Scientific Editorial Board—a multidisciplinary team specializing in:

• Solvent chemistry and formulation
• Analytical method validation
• Quality control for research reagents
• Regulatory compliance for laboratory products

Important Clarification: This acknowledgment recognizes scientific standards and institutional guidelines that inform our processes. It does not imply product endorsement, sponsorship, or affiliation with referenced organizations.

Ordering & Support

Available Products

Volume

Catalog #

Price

10 mL

AAC-010

[Price]

30 mL

AAC-030

[Price]

50 mL

AAC-050

[Price]

100 mL

AAC-100

[Price]

Bulk Discounts: Available for orders of 10+ bottles Custom Volumes: Contact us for institutional or custom requirements

How to Order

Online Ordering: Visit www.yourcompany.com

• Browse catalog
• Add items to cart
• Secure checkout
• Immediate order confirmation

Email Orders: Send to: orders@yourcompany.com Include: Product code, quantity, shipping address, PO number (if applicable)

Phone Orders: Call: +1-XXX-XXX-XXXX Hours: Monday-Friday, 8 AM - 6 PM EST

Shipping Information

Standard Shipping:

• Delivery: 3-5 business days
• Secure packaging prevents breakage
• Tracking number provided
• Signature may be required

Expedited Options:

• Next-day delivery available
• 2-day delivery available
• Saturday delivery in select areas

International Shipping:

• Available to most countries
• All necessary export documentation provided
• Customs support included
• Delivery time varies by destination

What's Included

With every order: ✓ Acetic Acid 0.6% Solution in sealed amber bottle ✓ Certificate of Analysis with batch data ✓ Safety Data Sheet (SDS) ✓ Product specification sheet ✓ Storage and handling guidelines ✓ Technical support access

Customer Support

Technical Assistance

Our scientific team provides expert support:

Application Guidance:

• Peptide reconstitution troubleshooting
• HPLC method development assistance
• Protocol optimization recommendations
• Alternative applications consultation

Product Information:

• Detailed specifications
• Compatibility with specific applications
• Custom formulation possibilities
• Bulk ordering arrangements

Quality Questions:

• COA interpretation
• Batch-specific data requests
• Stability information
• Storage recommendations

Contact Support

📧 Email: support@yourcompany.com (response within 24 hours) 📞 Phone: +1-XXX-XXX-XXXX (immediate assistance) 💬 Live Chat: Available on website during business hours 📄 FAQ: Comprehensive knowledge base at www.yourcompany.com/faq

Research Use Statement

CRITICAL NOTICE: This product is manufactured exclusively for laboratory research applications by qualified personnel in appropriate facilities.

NOT FOR: ❌ Human consumption or administration ❌ Veterinary use or animal treatment ❌ Pharmaceutical manufacturing (without GMP compliance) ❌ Food, beverage, or dietary applications ❌ Cosmetic or personal care products ❌ Any use outside controlled research settings

USER RESPONSIBILITIES: Purchasers certify they will: ✓ Use product only in appropriate laboratory settings ✓ Follow all institutional safety protocols ✓ Comply with local, state, and federal regulations ✓ Properly train personnel in chemical handling ✓ Dispose of waste according to regulations ✓ Maintain proper documentation and records

Quality Guarantee: We stand behind the quality of every product. If you experience any issues with purity, sterility, or performance, contact us immediately for resolution.

Legal Disclaimer: This product is for research use only. All information provided is for educational purposes based on established scientific standards. Users assume full responsibility for proper use, handling, and disposal in accordance with applicable regulations.